Drug & Alcohol Testing Services
From one-time partnerships to full management services, we handle compliance so you can focus on your business.

Your partner for nationwide testing services

- Affordable rates
- Walk-ins allowed
- Observed collections
- After hours drug screening
- Exceptional customer service
- Multilingual options
Our Testing Services
With our comprehensive drug and alcohol testing services, you can better concentrate on other aspects of your company's operations.
Full Management Service
You'll have access to a user-friendly online portal where we'll securely store all your paperwork, eliminating the hassle of dealing with piles of paper. Our team of experts will take care of everything for you, ensuring that your company always stays compliant with the rules.
DOT
Drug Testing
$69DOT
Alcohol Testing
$50Who would benefit from our services?
DOT regulated companies
DOT Regulated Companies that cover “ safety-sensitive” employees.
- Federal Motor Carrier Safety Administration (FMCSA)
- Federal Aviation Administration (FAA)
- Federal Railroad Administration (FRA)
- Federal Transit Association (FTA)
- Pipeline and Hazardous Materials Safety Administration (PHMSA)
- United States Coast Guard (USCG)
Other industries & cases
Employers in industries or roles that are not subject to DOT regulations. These may include:
- Private Sector Employers
- Legal or Judicial Industry
- Government Agencies
- Healthcare Facilities
- Educational Institutions
- Construction and Manufacturing Industries
Random Testing Program
Who must enroll?
If your employees perform safety-sensitive functions in industries regulated by the Department of Transportation (DOT), they are required to participate in random drug testing to ensure safety and compliance with DOT regulations.
Our random program is available for companies of all sizes. Our random selection software allows you to oversee each aspect of the process, from the first stage of random drug test selection through the moment the system sends you results.

If you are an owner-operator, you are required to be enrolled in a consortium random drug testing program. As a Route One Drug Test client enrolled in our random consortium program, we will help you to meet Clearinghouse requirements.

Smaller DOT-regulated employers may also choose to enroll in a consortium random drug testing program for easy compliance.
Larger DOT-regulated employers typically request a stand-alone DOT random testing program.
Advantages of partnering with us
Online Management Platform

By signing up, you'll gain access to our free online management portal to schedule tests, securely access results, and manage your testing process with ease. Ensure a safe and compliant workplace today!
Frequently Asked Questions
How often can an employee be selected for random testings?
In a truly random selection process, a high probability exists that some employees will be selected several times while others may never be selected.
Why? Because after each selection, the employee’s name is returned to the same pool, and he or she becomes just as likely as anyone else to be selected next time. Each employee shall have an equal chance of being tested each time selections are made.
What happens to me when I test positive or refuse to test?
When you test positive or refuse a test, you are not permitted to perform safety-sensitive duties for any DOT-regulated employer until you have seen a Substance Abuse Professional (SAP) and successfully completed the return-to-duty process, which includes a Federal return-to-duty drug and/or alcohol test. Working in a safety-sensitive position before successfully completing the return-to-duty process is a violation of the regulations.
What happens if I currently take medication that might interfere with my drug test results?
The laboratory that tests your specimen will send your test results to a Medical Review Officer (MRO) and if your result is positive the MRO will call you regarding your test result. The MRO will discuss with you any possible medication you are taking which could affect the outcome of the drug test. If there is a prescription the MRO will ask for the prescription information and pharmacy and verify this. If everything checks out your test result will have a final result of negative.
Can random testing be substituted for required follow-up testing?
No, random testing cannot typically be substituted for required follow-up testing. Random testing and follow-up testing serve different purposes within a drug testing program.
While both types of testing aim to ensure adherence to drug and alcohol policies and promote safety, they serve distinct purposes and cannot be used interchangeably. Random testing helps to deter substance use among all employees, while follow-up testing is targeted toward individuals with a history of substance abuse or positive test results to support their recovery and ensure ongoing compliance.
From the Blog
Read more
