Route One Drug Test
simplifying compliance
Our Mission
Our goal is to provide precise and trustworthy drug and alcohol testing services. We are committed to accuracy and professionalism while valuing confidentiality and adhering to regulatory standards.
We understand the importance of drug and alcohol testing in maintaining a safe and productive workplace environment. That is why we strive to offer our clients an exceptional service that is convenient and cost-effective.
Our value ads
30,000+ collection sites nationwide
Access to more than 30,000 testing locations allows you and your drivers to choose the most convenient routes, simplifying the testing process and seamlessly integrating it into your company's workflow.

Certified laboratories, collectors and technicians
Benefit from the assurance of compliance with industry standards and regulatory requirements through our SAMHSA-certified laboratories, urine collectors, and breath alcohol technicians. By entrusting your drug testing needs to us, you gain access to accurate and reliable results, while maintaining legal compliance.

Certified MRO reporting of results
The full-time Medical Review Officer results reporting through fax, email, and web ensures prompt and accurate communication of crucial information. We provide expert interpretation of test results while offering flexibility and convenience, catering to diverse operational needs.

Cutting-edge- web-based technology
By opting for our full management service you gain access to the web-based management portal for efficient program management, record-keeping, random selections, and reporting. Experience streamlined processes and enhanced accessibility, allowing for seamless management of your drug testing programs.

Multilingual consultation and support
You will be provided with ongoing consultations and administrative support, available in English, Romanian, and Russian. Our experienced team offers expert guidance and assistance, addressing any questions or concerns clients may have in their native language.

Walk-ins are welcome
By accommodating walk-ins at our own locations we ensure that clients can undergo testing whenever it's most convenient for them, reducing waiting times and minimizing disruptions to their schedules.

Frequently Asked Questions
How often can an employee be selected for random testing?
In a truly random selection process, a high probability exists that some employees will be selected several times while others may never be selected.
Why? Because after each selection, the employee’s name is returned to the same pool, and he or she becomes just as likely as anyone else to be selected next time. Each employee shall have an equal chance of being tested each time selections are made.
What happens to me when I test positive or refuse to test?
When you test positive or refuse a test, you are not permitted to perform safety-sensitive duties for any DOT-regulated employer until you have seen a Substance Abuse Professional (SAP) and successfully completed the return-to-duty process, which includes a Federal return-to-duty drug and/or alcohol test. Working in a safety-sensitive position before successfully completing the return-to-duty process is a violation of the regulations.
What happens if I currently take medication that might interfere with my drug test results?
The laboratory that tests your specimen will send your test results to a Medical Review Officer (MRO) and if your result is positive the MRO will call you regarding your test result. The MRO will discuss with you any possible medication you are taking which could affect the outcome of the drug test. If there is a prescription the MRO will ask for the prescription information and pharmacy and verify this. If everything checks out your test result will have a final result of negative.
Can random testing be substituted for required follow-up testing?
No, random testing cannot typically be substituted for required follow-up testing. Random testing and follow-up testing serve different purposes within a drug testing program.
While both types of testing aim to ensure adherence to drug and alcohol policies and promote safety, they serve distinct purposes and cannot be used interchangeably. Random testing helps to deter substance use among all employees, while follow-up testing is targeted toward individuals with a history of substance abuse or positive test results to support their recovery and ensure ongoing compliance.
Online Management Platform

By signing up, you'll gain access to our free online management portal to schedule tests, securely access results, and manage your testing process with ease. Ensure a safe and compliant workplace today!