DOT Drug Testing
Our approach to DOT Drug Testing
Our DOT Drug Testing Service is available to any employer within the U.S. Our collectors are trained and certified in accordance with the Department of Transportation procedures for drug and alcohol testing of safety-sensitive employees.
What we offer:
- Access to 30,000+ collection sites nationwide
- Certified, full-time, MRO reporting of results - via fax, e-mail, and or web reporting
- NHTSA Approved EBTs (Evidential Breath Testing devices)
- SAMHSA-certified laboratories, certified urine collectors, and certified breath alcohol technicians
- Cutting-edge web-based technology used for management, record-keeping, random selections, and test results reporting
- Ongoing consultation and administrative support in English, Romanian, and Russian

Understanding the DOT Drug Testing
The U.S. Department of Transportation (DOT), as a federal agency, established regulations requiring drug testing for safety-sensitive transportation employees.
DOT mandates a 5-Panel Drug Test:
- Amphetamines
- Cocaine
- Marijuana (THC)
- Opioids
- Phencyclidine (PCP)
The testing process always consist of 3 steps:
- Collection
- Testing at the Laboratory
- Review by the Medical Officer
*The testing process follows strict procedures outlined in the regulations.
Reasons for DOT Drug Testing
Enroll nowPre-employment
Employers MUST receive a negative drug screen result from an HHS-approved lab and should be reviewed by a certified MRO prior to allowing that employee to perform safety-sensitive duties.
Random
Random drug testing selections should be conducted by using a scientifically valid method. Random means that every covered employee has an equal chance to be tested. Each DOT agency determines the annual random testing rates and these rates are subject to change each year.
Post-accident
When a covered employee has an accident that meet certain criteria, he or she must take a drug test to determine if drug use contributed to the accident. The rules of each operating agency define exactly which types of accidents require a post-accident test. The drug tests should be completed within 32 hours of the accident.
Reasonable suspicion
When a trained supervisor notices unusual behavior in an employee that suggests drug or alcohol use, the supervisor must direct that employee to take a drug test. Only supervisors who have completed the drug and alcohol awareness training may request a reasonable suspicion test
Return to Duty
Only completed on employees returning to safety-sensitive duties following a positive drug or alcohol test and who have completed a treatment or education program. All return-to-duty tests must be directly observed.
Follow-up
The testing schedule is always determined by the SAP. DOT rules require a minimum of 6 “surprise” tests over 12 months. All follow-up tests must be directly observed.
DOT Regulated Agencies
Federal Motor Carrier Safety Administration
Federal Aviation Administration
Federal Railroad Administration
Federal Transit Administration
Pipeline and Hazardous Materials Safety Administration
United States Coast Guard
*If you are not sure if you are subject to the DOT Drug & Alcohol Testing Regulations, the Department of Transportation created a guide to help you.

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